Cloning and expression of acd genes of FMDV

RT-PCR amplification of VIA [3D] gene and protease [3C] using its primers. Legation of the PCR product with the cloning vector [using pET system]. Expression of the recombinant plasmid in E. coli expression host. Use of the expressed VIA protein in diagnosis of cattle infected with any of the 7 serotypes of FMD virus with the help of ELISA

Differentiation between infected repeatedly vaccinated and free animal with fmd virus using bio-engineered VIA

Sera from infected, vaccinated and negative cattle were tested using 3D ELISA to allow each category of animals to be clearly differentiated based on the presence of antibody to 3D antigen. The assay can recognize infected animals in countries where FMD outbreaks have occurred, specially if ring vaccination has been performed, also at the same time vaccinated animals can become infected without apparent clinical symptoms. 3D ELISA test is simple and rapid to discriminate between antibodies elicited by FMD infection or by vaccination

antigenic variation of foot and mouth disease virus of the last three isolates in Egypt

Three FMD viral isolates had been isolated from different outbreaks at three governorates [Sharquia, Suez, and Agga locality] during 1972, 1987, and 1993 from cattle respectively. The isolates were tested by coinplement fixation test [CFT] angainst the seven serotypes and the results revealed that all strains were of the type [O] FMD virus. The studied isolates were adapted after the 3[rd] and 4[th] passages to BEK cell cultures, they yielded infective titers ranging between 4 and 5.5 log 10 TCID[50] and complement fixing titers between 1.37-1.75 log[10] CFU/ml. Antigenic relationship and dominating state studies for the three isolates using the crosswise C.F.T. revealed that isolates [O[1]/72 and O[1] /87] and [O[1]/72] and O[1]/93] showing a little antigenic variation and can be classified as [Subtype similar but still different] and have a percentage between [32% to 70%] while isolates [O[1]/87 and O[1] /93] classified as [Subtype Similar] and have percentage between [70%-100%]. The dominance determination for isolated strains showed that isolate strain [O[1]/87] is dominating strain [O[1]/72 and O[1] /93] and strain O[1]/72 is dominating strain O[1]/93. The nucleotide sequence data of VPI of the isolated strains [O[1]/72, O[1]/87 and O[1]/93] revealed that the homology% FMD virus [O[1]/72, O[1] 87 with O[1]/93] were 86% and 88% respectively, while the homology% between O/87 and O/93 was 94%. This indicates a closer relationship between O[1]/87 and O[1]/93 isolates thorn to O[1]/72. The variation of the results between CF test and nucleotide sequence homology may be attnbuted to the fact that CF test is carried on the whole FMDV while the nucleotide sequence homology carried out on the level of VPI gene. However, this difference is not of a significant value that necessitates the change of the already applicable vaccinal strain